4JMF
Crystal structure of ExoT (residues 28 -77)- SpcS complex from Pseudomonas aeruginosa at 2.1 angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU ULTRAX 18 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-08-05 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 78.322, 78.322, 194.221 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.482 - 2.099 |
R-factor | 0.202 |
Rwork | 0.198 |
R-free | 0.24310 |
Structure solution method | MIR |
RMSD bond length | 0.007 |
RMSD bond angle | 1.172 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 50.000 | 2.260 |
High resolution limit [Å] | 2.099 | 3.590 | 2.090 |
Number of reflections | 21244 | ||
Completeness [%] | 99.0 | 99 | 95 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 295 | 28% PEGMME 5000, 0.2M ammonium sulphate, pH 6.5, vapour diffusion, temperature 295K, VAPOR DIFFUSION |