4JI0

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	403.742, 403.742, 174.338
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.528 - 3.492
R-factor	0.1692
Rwork	0.167
R-free	0.21160
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	1.044
Data scaling software	XSCALE
Phasing software	PHENIX (dev_1119)
Refinement software	PHENIX (dev_1119)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.700
High resolution limit [Å]	3.490	10.270	3.490
Rmerge	0.077	0.035	0.763
Number of reflections	179519	7247	27843
<I/σ(I)>	11.7	35.49	1.71
Completeness [%]	99.1	95.4	96.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	MPD , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K