4JHK
Crystal structure of Danio rerio SLIP1 in complex with SLBP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | PSI PILATUS 6M |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 70.650, 70.650, 242.550 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.069 - 2.507 |
R-factor | 0.1948 |
Rwork | 0.192 |
R-free | 0.24320 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.111 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 43.070 |
High resolution limit [Å] | 2.500 |
Number of reflections | 13244 |
Completeness [%] | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.17 M K/Na-tartrate, 0.085 M Na-citrate pH 5.6, 1.7 M ammonium sulfate and 15% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |