4JAB
U/G Wobble Base Pair in a RNA Duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97895 |
| Spacegroup name | H 3 |
| Unit cell lengths | 41.681, 41.681, 60.014 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.930 - 2.230 |
| R-factor | 0.15648 |
| Rwork | 0.152 |
| R-free | 0.22898 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 246d |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.093 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.930 | 2.310 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.066 | 0.330 |
| Number of reflections | 1723 | |
| Completeness [%] | 91.0 | 90.1 |
| Redundancy | 2.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
