4J9O
Human DNA polymerase eta-DNA ternary complex: primer extension after a T:G mispair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-01-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 |
| Unit cell lengths | 99.490, 99.490, 81.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.091 - 2.597 |
| R-factor | 0.2098 |
| Rwork | 0.208 |
| R-free | 0.23360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mr2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.102 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 | 2.670 |
| High resolution limit [Å] | 2.597 | 2.910 | 2.600 |
| Rmerge | 0.124 | 0.436 | 0.772 |
| Number of reflections | 14144 | ||
| <I/σ(I)> | 12.4 | 4.09 | 2.36 |
| Completeness [%] | 99.5 | 99.9 | 98.7 |
| Redundancy | 4.8 | 4.87 | 4.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG2K-MME, KCl, MES, pH 6.5, vapor diffusion, hanging drop, temperature 298K |
