4IZJ
Crystal structure of yellowtail ascites virus VP4 protease with a wild-type active site reveals acyl-enzyme complexes and product complexes.
Replaces: 3R0BExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-08-05 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.610, 64.330, 187.740 |
| Unit cell angles | 90.00, 95.80, 90.00 |
Refinement procedure
| Resolution | 64.300 - 2.500 |
| R-factor | 0.18167 |
| Rwork | 0.178 |
| R-free | 0.24397 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pnm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.900 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.300 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.088 | 0.278 |
| Number of reflections | 33009 | |
| <I/σ(I)> | 10.5 | 3.8 |
| Completeness [%] | 95.9 | 80.7 |
| Redundancy | 3.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 296 | 35% PEG2000, 0.3M Magnesium Chloride, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
