4IY8
Bmlp3 - P21 crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-06 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.930, 124.410, 67.890 |
Unit cell angles | 90.00, 114.80, 90.00 |
Refinement procedure
Resolution | 33.340 - 2.360 |
R-factor | 0.17578 |
Rwork | 0.173 |
R-free | 0.25920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4efp |
RMSD bond length | 0.015 |
RMSD bond angle | 1.715 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.400 | 2.460 |
High resolution limit [Å] | 2.360 | 2.360 |
Rmerge | 0.082 | 0.636 |
Number of reflections | 35189 | |
<I/σ(I)> | 15.7 | 2.74 |
Completeness [%] | 99.5 | 99.7 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 30% PEG MME 550, 0.1 M CaCl2, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |