4IDZ

Crystal structure of the human fat mass and obesity associated protein (FTO) in complex with N-oxalylglycine (NOG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-12
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	H 3
Unit cell lengths	140.412, 140.412, 83.447
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.540 - 2.460
Rwork	0.194
R-free	0.24470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lfm
RMSD bond length	0.009
RMSD bond angle	1.178
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.550
High resolution limit [Å]	2.460	5.300	2.460
Rmerge	0.080	0.047	0.693
Number of reflections	22286
<I/σ(I)>	17.2	24.1	2.2
Completeness [%]	98.6	96.8	99.9
Redundancy	5.1	4.9	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	14% PEG 3350, 85mM trisodium citrate pH5.6, 10% glycerol, 1mM NiCl2, 3mM NOG, 5mM 6-methyladenosine, VAPOR DIFFUSION, HANGING DROP, temperature 293K