4IA4
Structure of the spinach aquaporin SoPIP2;1 at pH 6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9395 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 178.227, 104.190, 66.523 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 90.110 - 3.100 |
R-factor | 0.23733 |
Rwork | 0.237 |
R-free | 0.24281 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z98 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.584 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 104.260 | 3.270 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.099 | 0.667 |
Number of reflections | 22668 | |
<I/σ(I)> | 11.1 | 1.6 |
Completeness [%] | 98.2 | 99.6 |
Redundancy | 3.9 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 278 | 22% PEG400, 0.1M NaCitrate, 0.05M NaCl, 0.02M MgCl2, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 278K |