4I9Q
Crystal structure of the ternary complex of the D714A mutant of RB69 DNA polymerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-11-20 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.781, 119.412, 146.016 |
Unit cell angles | 90.00, 90.26, 90.00 |
Refinement procedure
Resolution | 38.403 - 2.300 |
R-factor | 0.2537 |
Rwork | 0.252 |
R-free | 0.28710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ngi |
RMSD bond length | 0.012 |
RMSD bond angle | 0.781 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1227)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.117 |
High resolution limit [Å] | 2.300 |
Number of reflections | 110655 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 10% PEG 8000, 0.2 M (NH4)2SO4 and 0.1 M sodium citrate (pH 5.6), VAPOR DIFFUSION, HANGING DROP, temperature 298K |