4I5J
PP2A PR70 Holoenzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2006-08-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.10 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 67.610, 67.610, 139.110 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.849 - 2.091 |
R-factor | 0.197 |
Rwork | 0.194 |
R-free | 0.25220 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.122 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.180 |
High resolution limit [Å] | 2.090 | 2.090 |
Number of reflections | 21335 | |
<I/σ(I)> | 10.9 | 1.3 |
Completeness [%] | 94.9 | 61.2 |
Redundancy | 4.7 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | 50:50 (0.1 M NaH2PO4/0.1 M K2HPO4, 0.1 M MES, 2 M NaCl) 8 mg/ml protein , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |