4HSD
Crystal structure of a new form of plant lectin from Cicer arietinum at 2.45 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-05-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 3 |
| Unit cell lengths | 80.481, 80.481, 69.177 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 69.700 - 2.450 |
| R-factor | 0.25775 |
| Rwork | 0.256 |
| R-free | 0.28853 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v6n |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.974 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.700 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Number of reflections | 17896 | |
| Completeness [%] | 97.0 | 86.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.2M Magnesium acetate tetra hydrate, 0.1M sodiun cacodylate trihydrate, 20% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
