4HO7
Crystal structure of eukaryotic HslV from Trypanosoma brucei
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-08 |
Detector | ADSC QUANTUM 210r |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 105.673, 111.062, 116.953 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.730 - 2.601 |
R-factor | 0.21164 |
Rwork | 0.210 |
R-free | 0.24805 |
Structure solution method | MIR |
RMSD bond length | 0.014 |
RMSD bond angle | 1.544 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.600 |
Number of reflections | 21375 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris-HCl pH 8.5, 3.5M Sodiumformate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |