4HNM
Crystal structure of human catenin-beta-like 1 56 kDa fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-E |
Synchrotron site | APS |
Beamline | 21-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 94.970, 94.970, 172.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.245 - 2.900 |
R-factor | 0.2122 |
Rwork | 0.207 |
R-free | 0.25760 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.687 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.245 | 34.526 | 2.950 |
High resolution limit [Å] | 2.800 | 8.850 | 2.800 |
Rmerge | 0.039 | 0.775 | |
Number of reflections | 20205 | ||
<I/σ(I)> | 23.4 | 14.9 | 1 |
Completeness [%] | 99.9 | 98.2 | 100 |
Redundancy | 19.8 | 16.3 | 19.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 2000, Tris 8.0, KBr, vapor diffusion, hanging drop, temperature 298K |