4HM9
Crystal structure of full-length human catenin-beta-like 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-E |
Synchrotron site | APS |
Beamline | 21-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.990, 116.360, 137.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.618 - 3.100 |
R-factor | 0.1772 |
Rwork | 0.174 |
R-free | 0.22530 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.597 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.145 | 46.145 | 2.530 |
High resolution limit [Å] | 2.399 | 7.590 | 2.399 |
Rmerge | 0.035 | 0.035 | |
Number of reflections | 31661 | ||
<I/σ(I)> | 6.1 | 9.9 | 0.2 |
Completeness [%] | 100.0 | 99.6 | 99.9 |
Redundancy | 6.7 | 6.3 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 2000, Tris 8.0, KBr, vapor diffusion, hanging drop, temperature 298K |