4H6Y
Crystal structure of the DH-PH-PH domain of FARP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97915 |
Spacegroup name | P 32 |
Unit cell lengths | 142.237, 142.237, 105.180 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.574 - 4.090 |
R-factor | 0.2902 |
Rwork | 0.289 |
R-free | 0.31730 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.718 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 4.170 |
High resolution limit [Å] | 4.090 | 11.090 | 4.090 |
Rmerge | 0.107 | 0.069 | 0.506 |
Number of reflections | 18762 | ||
<I/σ(I)> | 10 | ||
Completeness [%] | 99.5 | 97.4 | 97.7 |
Redundancy | 7.7 | 7.1 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 100 mM MES, 0.2 M Mg formate, 14% PEG3350, pH 6.4, vapor diffusion, hanging drop, temperature 293K |