4H33
Crystal structure of a voltage-gated K+ channel pore module in a closed state in lipid membranes, tetragonal crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0330 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 53.370, 53.370, 108.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 16.400 - 3.100 |
R-factor | 0.2845 |
Rwork | 0.281 |
R-free | 0.30500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eff 2atk 2A79 and 1ORQ |
RMSD bond length | 0.010 |
RMSD bond angle | 1.010 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.132 | 0.920 |
Number of reflections | 2994 | |
<I/σ(I)> | 18.6 | 2.45 |
Completeness [%] | 94.4 | 75 |
Redundancy | 7.1 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 293 | 26-30% (v/v) PEG-MME-550, 0.5 M to 0.6 M ammonium sulfate, and 50 mM ADA, Lipidic Cubic Phase (LCP) , pH 7.0, temperature 293K |