4H0K
Mutant m58h of Nostoc sp cytochrome c6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2005-05-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.95371 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.370, 60.370, 95.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.260 - 1.950 |
| R-factor | 0.21599 |
| Rwork | 0.213 |
| R-free | 0.26802 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gyd |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.964 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0027) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.260 | |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 14317 | |
| Completeness [%] | 99.6 | 99.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1M Tris, 2.5M Ammonium sulphate, 0.1M Lithium sulphate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
