4GZJ
Active-site mutant of potato endo-1,3-beta-glucanase in complex with laminaratriose and laminaratetrose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-02-04 |
Detector | MAR555 FLAT PANEL |
Wavelength(s) | 0.81480 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.307, 49.202, 57.431 |
Unit cell angles | 90.00, 98.84, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.550 |
R-factor | 0.1515 |
Rwork | 0.151 |
R-free | 0.18060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gzi |
RMSD bond length | 0.017 |
RMSD bond angle | 1.644 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.091 | 0.680 |
Number of reflections | 44391 | |
<I/σ(I)> | 15.3 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 292 | 0.1 M sodium citrate, 0.2 M ammonium acetate, 24% PEG 8000, 0.4 mM laminarahexose, streak seeding, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 292K |