4GYO
Crystal Structure of Rap Protein Complexed with Competence and Sporulation Factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 86.725, 86.725, 225.805 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.000 - 2.160 |
R-factor | 0.1913 |
Rwork | 0.190 |
R-free | 0.22510 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.480 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 |
High resolution limit [Å] | 2.160 | 2.160 |
Number of reflections | 47237 | |
Completeness [%] | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 18 mM Tris (pH 8.0), 133 mM KCl, 4 mM DTT, 294 mM magnesium chloride, 2% benzamidine hydrochloride, 9% PEG 3000, 100 mM sodium cacodylate (pH 6.4), VAPOR DIFFUSION, HANGING DROP, temperature 293K |