4GRU

crystallographic and biological characterization of N- and C- terminus mutants of human MIF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2010-06-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 31 2 1
Unit cell lengths	95.562, 95.562, 103.778
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.920
R-factor	0.2009
Rwork	0.201
R-free	0.23010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	human MIF
RMSD bond length	1.230
RMSD bond angle	0.005
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.920
Rmerge	0.096
Number of reflections	42321
<I/σ(I)>	22.6
Completeness [%]	99.8
Redundancy	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2.0M ammonium sulfate, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K