4GP6
Polynucleotide kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 130 |
Detector technology | PIXEL |
Collection date | 2012-03-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.10 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.690, 71.560, 118.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.780 - 2.100 |
R-factor | 0.181 |
Rwork | 0.177 |
R-free | 0.22820 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.095 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.600 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 22214 | |
Completeness [%] | 98.9 | 92.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100mM HEPES, 200mM MgCl2, 30% PEG 400, 2mM ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |