4G59
Crystal structure of the murine cytomegalovirus MHC-I homolog m152 with ligand RAE-1 gamma
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2010-03-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 193.443, 99.800, 68.609 |
Unit cell angles | 90.00, 100.72, 90.00 |
Refinement procedure
Resolution | 47.516 - 2.440 |
R-factor | 0.2032 |
Rwork | 0.201 |
R-free | 0.24510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PBD entries 2O5N 1jfm |
RMSD bond length | 0.009 |
RMSD bond angle | 1.269 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.440 | 2.440 |
Rmerge | 0.070 | 0.080 |
Number of reflections | 45215 | |
<I/σ(I)> | 2.8 | |
Completeness [%] | 94.7 | 96.4 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 15% PEG3350, 0.2M KCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |