4FUO
Structural basis for Zn2+-dependent intercellular adhesion in staphylococcal biofilms
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 121.105, 34.619, 49.369 |
Unit cell angles | 90.00, 103.11, 90.00 |
Refinement procedure
Resolution | 25.980 - 1.970 |
R-factor | 0.2095 |
Rwork | 0.207 |
R-free | 0.25740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
High resolution limit [Å] | 1.960 | 4.220 | 1.960 |
Rmerge | 0.083 | 0.063 | 0.537 |
Number of reflections | 13864 | ||
<I/σ(I)> | 14.7 | ||
Completeness [%] | 95.6 | 94.6 | 75.9 |
Redundancy | 5.2 | 5.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | batch | 7.9 | 293 | 0.06 M Postassium Thiocyanate, 0.1 M Tris pH 7.9, 28% PEG MME 2000, 2% PEG 3350, batch, temperature 293K |