4FP9

Human MTERF4-NSUN4 protein complex

Experimental procedure

Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	78.760, 82.270, 507.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.900
R-factor	0.2295
Rwork	0.229
R-free	0.24610
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	0.900
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	BUSTER-TNT (BUSTER 2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		3.000
High resolution limit [Å]	2.900	40.000	2.900
Rmerge	0.105	0.020	0.977
Number of reflections	74514	11	7096
<I/σ(I)>	11.58	45.94	1.76
Completeness [%]	99.8	27.5	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	294	100 mM Bis-Tris [pH 5.5], 400 mM ammonium sulfate, 20-22 % (w/v) polyethylene glycol 3350, and 2 mM dithiothreitol, VAPOR DIFFUSION, temperature 294K