4F0Y
Crystal structure of aminoglycoside antibiotic 6'-N-acetyltransferase AAC(6')-IG from Acinetobacter haemolyticus, apo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-07 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 1.541780 |
Spacegroup name | P 1 |
Unit cell lengths | 37.963, 44.018, 46.576 |
Unit cell angles | 83.19, 87.44, 72.65 |
Refinement procedure
Resolution | 24.073 - 2.560 |
R-factor | 0.1839 |
Rwork | 0.181 |
R-free | 0.24010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s3z |
RMSD bond length | 0.004 |
RMSD bond angle | 0.655 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.610 |
High resolution limit [Å] | 2.560 | 2.560 |
Number of reflections | 8914 | |
<I/σ(I)> | 8.205 | 2.095 |
Completeness [%] | 96.9 | 94.6 |
Redundancy | 2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.2 M magnesium chloride, 0.1 M Bis-Tris, pH 5.5, 25% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |