4EV5
Crystal structure of copper amine oxidase-1 from Hansenula polymorpha in complex with benzylamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.239, 233.706, 105.045 |
| Unit cell angles | 90.00, 96.57, 90.00 |
Refinement procedure
| Resolution | 48.900 - 2.250 |
| R-factor | 0.17212 |
| Rwork | 0.169 |
| R-free | 0.22989 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oov |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.951 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.089 | 0.324 |
| Number of reflections | 232595 | |
| <I/σ(I)> | 9.8 | 1.8 |
| Completeness [%] | 98.8 | 97.7 |
| Redundancy | 4.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 8% PEG8000, 0.22 M potassium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
