4EL9
Structure of N-terminal kinase domain of RSK2 with afzelin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9712 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.119, 40.860, 83.867 |
| Unit cell angles | 90.00, 114.64, 90.00 |
Refinement procedure
| Resolution | 16.710 - 1.550 |
| R-factor | 0.2264 |
| Rwork | 0.200 |
| R-free | 0.22640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ubd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.939 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.000 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 43153 | |
| <I/σ(I)> | 23.2 | 2 |
| Completeness [%] | 96.8 | 76.9 |
| Redundancy | 4.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 30% Jeffamine ED2003, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
