4EEI

Crystal Structure of Adenylosuccinate Lyase from Francisella tularensis Complexed with AMP and Succinate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 43 21 2
Unit cell lengths	88.759, 88.759, 254.724
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.331 - 1.921
R-factor	0.169
Rwork	0.168
R-free	0.19900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 1C3U
RMSD bond length	0.007
RMSD bond angle	0.992
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((phenix.refine: 1.7.3_920))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.920	1.920
Rmerge	0.105	0.798
Number of reflections	78595
<I/σ(I)>	7.5	4
Completeness [%]	100.0	100
Redundancy	15.9	15.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	1.0M succinic acid pH7.0, 0.1M HEPES pH7.0, 1% PEG MME 2000, VAPOR DIFFUSION, SITTING DROP, temperature 289K