4E8F
Structural Basis for the Activity of a Cytoplasmic RNA Terminal U-transferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9173 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.150, 101.210, 113.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.790 - 2.600 |
R-factor | 0.1942 |
Rwork | 0.192 |
R-free | 0.23880 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.970 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.9.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 56.800 |
High resolution limit [Å] | 2.600 |
Number of reflections | 29430 |
Completeness [%] | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 15% (v/v) glycerol, 25.5% (w/v) PEG4000, 170mM ammonium acetate, 85mM tri-sodium citrate, pH 5.6 with 1% taurine, VAPOR DIFFUSION, SITTING DROP, temperature 293K |