4E11
Crystal structure of kynurenine formamidase from Drosophila melanogaster
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0750 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 75.771, 47.085, 85.683 |
Unit cell angles | 90.00, 90.34, 90.00 |
Refinement procedure
Resolution | 42.840 - 2.000 |
R-factor | 0.22743 |
Rwork | 0.225 |
R-free | 0.27344 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pbl |
RMSD bond length | 0.020 |
RMSD bond angle | 1.663 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.150 | 0.620 |
Number of reflections | 19948 | |
<I/σ(I)> | 23.3 | |
Redundancy | 7 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |