4DPZ
Crystal structure of human HRASLS2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97949 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.738, 37.078, 45.423 |
Unit cell angles | 90.00, 113.38, 90.00 |
Refinement procedure
Resolution | 20.820 - 1.250 |
R-factor | 0.15147 |
Rwork | 0.150 |
R-free | 0.17884 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dot |
RMSD bond length | 0.013 |
RMSD bond angle | 1.657 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.290 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.047 | 0.285 |
Number of reflections | 34298 | |
<I/σ(I)> | 22 | 3.7 |
Completeness [%] | 98.0 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 1.1 ammonium tartrate dibasic, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |