4DNE
Crystal structure of a triple-mutant of streptavidin in complex with desthiobiotin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-04 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9334 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 46.720, 93.782, 104.413 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.860 - 1.880 |
R-factor | 0.18323 |
Rwork | 0.181 |
R-free | 0.21897 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mk5 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.382 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MERLOT |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.121 | 0.410 |
Number of reflections | 19097 | |
Completeness [%] | 99.5 | 96.7 |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 10 mM MgSO4, 50 mM Na caodylate, 2M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |