4DIQ
Crystal Structure of human NO66
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-10 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.99190 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 78.674, 149.550, 105.311 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.460 - 2.400 |
R-factor | 0.18702 |
Rwork | 0.185 |
R-free | 0.23045 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.683 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.637 | |
Number of reflections | 49295 | |
<I/σ(I)> | 22.8 | 2.1 |
Completeness [%] | 99.9 | 98.6 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.05M (NH4)2SO4, 0.05M BIS-TRIS pH 6.5, 30% pentaerythritol ethoxylate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |