4DH4
Macrophage migration inhibitory factor Toxoplasma gondii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2009-02-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97630 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 58.730, 58.730, 183.971 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.000 - 1.820 |
| R-factor | 0.19486 |
| Rwork | 0.193 |
| R-free | 0.23107 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.066 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 1.920 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.083 | 0.395 |
| Number of reflections | 11318 | |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 1.82M ammonium sulphate, 100mM Tris pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
