4DFH
Crystal structure of cell adhesion molecule nectin-2/CD112 variable domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-06-01 |
| Detector | ENRAF-NONIUS |
| Wavelength(s) | 0.9783 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.673, 43.850, 56.075 |
| Unit cell angles | 90.00, 118.24, 90.00 |
Refinement procedure
| Resolution | 27.974 - 1.850 |
| R-factor | 0.1722 |
| Rwork | 0.171 |
| R-free | 0.19420 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.976 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 19484 | |
| Completeness [%] | 92.1 | 91.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.2M ammonium acetate, 0.1M Bis-Tris (pH 5.5), 25% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
