4CZT
Crystal structure of the kinase domain of CIPK23
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-26 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.250, 91.460, 207.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.262 - 2.300 |
R-factor | 0.1789 |
Rwork | 0.176 |
R-free | 0.23440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3h4j |
RMSD bond length | 0.009 |
RMSD bond angle | 1.122 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.300 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.180 | 1.150 |
Number of reflections | 61816 | |
<I/σ(I)> | 12.8 | 3 |
Completeness [%] | 99.0 | 99 |
Redundancy | 13.2 | 12.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 16 MM CHAPS 0.1 M HEPES PH 7.5, 3.5 M AMMONIUM SULFATE AS PRECIPITANT MIXED WITH THE PROTEIN AT 14 MG/ML USING MICROBATCH METHOD. |