4CTA
Competence or damage-inducible protein CinA from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.590, 93.770, 131.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.890 - 2.210 |
| R-factor | 0.22312 |
| Rwork | 0.220 |
| R-free | 0.27380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a9s |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.842 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.000 | 2.270 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.100 | 0.570 |
| Number of reflections | 46732 | |
| <I/σ(I)> | 11.2 | 2 |
| Completeness [%] | 99.4 | 99.2 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M SODIUM SULFATE, 0.1 M BIS-TRIS PROPANE, 20 % (W/V) PEG 3350 |
