4CSD
Structure of Monomeric Ralstonia solanacearum lectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-22 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.420, 75.910, 85.780 |
| Unit cell angles | 90.00, 102.23, 90.00 |
Refinement procedure
| Resolution | 41.920 - 1.350 |
| R-factor | 0.11721 |
| Rwork | 0.116 |
| R-free | 0.14875 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bt9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.619 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.920 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.040 | 0.150 |
| Number of reflections | 99018 | |
| <I/σ(I)> | 14.8 | 5.6 |
| Completeness [%] | 99.1 | 95.5 |
| Redundancy | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS HYDROCHLORIDE PH 8.5, 2M AMMONIUM SULFATE |
