4CF4
Mutagenesis of a Rhodobacteraceae L-haloacid dehalogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 287 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.090, 86.640, 105.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.970 - 2.140 |
R-factor | 0.20583 |
Rwork | 0.202 |
R-free | 0.28003 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yml |
RMSD bond length | 0.012 |
RMSD bond angle | 1.642 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.970 | 2.200 |
High resolution limit [Å] | 2.140 | 2.140 |
Rmerge | 0.060 | 0.580 |
Number of reflections | 23818 | |
<I/σ(I)> | 13.5 | 3.1 |
Completeness [%] | 98.9 | 98.9 |
Redundancy | 6.3 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | pH 8 |