4C99

Mouse ZNRF3 ectodomain in complex with mouse RSPO2 Fu1-Fu2 crystal form I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-18
Detector	DECTRIS PIXEL
Spacegroup name	P 21 21 21
Unit cell lengths	59.800, 77.206, 130.611
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.460 - 2.800
R-factor	0.2405
Rwork	0.236
R-free	0.32285
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.013
RMSD bond angle	1.656
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	66.000
High resolution limit [Å]	2.800
Rmerge	0.120
Number of reflections	15525
<I/σ(I)>	16
Completeness [%]	99.9
Redundancy	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1