4C7N
Crystal Structure of the synthetic peptide iM10 in complex with the coiled-coil region of MITF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-08 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 23.701, 33.265, 61.125 |
Unit cell angles | 90.00, 94.65, 90.00 |
Refinement procedure
Resolution | 60.920 - 2.100 |
R-factor | 0.22872 |
Rwork | 0.228 |
R-free | 0.25312 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.175 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.920 | 2.180 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.100 | 0.370 |
Number of reflections | 7041 | |
<I/σ(I)> | 15.6 | 6.6 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 12.6 | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 3.5 M NAFORMATE PH 7.0 |