4BWS
Crystal structure of the heterotrimer of PQBP1, U5-15kD and U5-52kD.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-27 |
| Detector | MARRESEARCH |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 144.180, 40.470, 168.171 |
| Unit cell angles | 90.00, 95.92, 90.00 |
Refinement procedure
| Resolution | 41.818 - 2.500 |
| R-factor | 0.2399 |
| Rwork | 0.238 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1syx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.192 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.270 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.240 | 0.610 |
| Number of reflections | 34203 | |
| <I/σ(I)> | 4.1 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
