4BWM
KlenTaq mutant in complex with a RNA/DNA hybrid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-04-27 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 143.192, 86.282, 63.094 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.985 - 1.749 |
R-factor | 0.17 |
Rwork | 0.168 |
R-free | 0.20760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rtv |
RMSD bond length | 0.007 |
RMSD bond angle | 1.089 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE: DEV_1411)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.340 | 1.850 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.080 | 1.120 |
Number of reflections | 79729 | |
<I/σ(I)> | 13.73 | 1.7 |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 6.59 | 6.62 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | HANGING DROP. 100MM TRIS PH8.5, 200MM MG-FORMATE, 20% PEG 8K MICROSEEDED |