4BOQ
Structure of OTUD2 OTU domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 60.670, 60.670, 86.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.727 - 1.470 |
| R-factor | 0.1721 |
| Rwork | 0.171 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3by4 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.065 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.320 | 1.550 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.070 | 0.620 |
| Number of reflections | 30915 | |
| <I/σ(I)> | 10.5 | 2 |
| Completeness [%] | 97.2 | 99.5 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.8M SODIUM PHOSPHATE MONOBASIC MONOHYDRATE, 0.1 M TRIS PH 7.5 |
