4BKS
von Hippel Lindau protein:ElonginB:ElonginC complex, in complex with (2S,4R)-1-ethanoyl-N-[[4-(1,3-oxazol-5-yl)phenyl]methyl]-4-oxidanyl-pyrrolidine-2-carboxamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 93.590, 93.590, 363.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.470 - 2.200 |
| R-factor | 0.1895 |
| Rwork | 0.188 |
| R-free | 0.22480 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | BUSTER (TNT) |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.120 | 1.500 |
| Number of reflections | 83354 | |
| <I/σ(I)> | 14.95 | 2.18 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.9 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M NA CACODYALATE PH 6.0, 0.2M MG ACETATE, 15%PEG3350, 5MM DTT |
