4BE3
crystal structure of the exolytic PL7 alginate lyase AlyA5 from Zobellia galactanivorans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-13 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.420, 93.910, 130.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.950 - 1.750 |
R-factor | 0.15744 |
Rwork | 0.156 |
R-free | 0.17802 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z42 |
RMSD bond length | 0.033 |
RMSD bond angle | 2.896 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.950 | 1.800 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.100 | 0.840 |
Number of reflections | 114168 | |
<I/σ(I)> | 16.53 | 2.3 |
Completeness [%] | 99.4 | 93.3 |
Redundancy | 8.47 | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 22% PEG 3350 AND 0.2 M SODIUM/POTASSIUM TARTRATE, pH 7 |