4BDP
CRYSTAL STRUCTURE OF BACILLUS DNA POLYMERASE I FRAGMENT COMPLEXED TO 11 BASE PAIRS OF DUPLEX DNA AFTER ADDITION OF TWO DATP RESIDUES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-05-15 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.241, 93.275, 106.374 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bdp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 25.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.047 * | 0.133 * |
| Total number of observations | 314981 * | |
| Number of reflections | 72444 | |
| Completeness [%] | 90.6 | 76.6 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.8 | 18 * | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 55 (%(v/v)sat) | |
| 3 | 1 | reservoir | MES | 100 (mM) | |
| 4 | 1 | reservoir | MPD | 2.5-7.0 (%(v/v)) | |
| 5 | 1 | reservoir | sodium azide | 0.02 (%(w/v)) |
