4B9S
Structure of the high fidelity DNA polymerase I with an oxidative formamidopyrimidine-dG DNA lesion outside of the pre-insertion site.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-03-24 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.066, 93.688, 105.604 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.840 - 1.730 |
R-factor | 0.18242 |
Rwork | 0.181 |
R-free | 0.20643 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4b9m |
RMSD bond length | 0.017 |
RMSD bond angle | 1.821 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.800 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.040 | 0.510 |
Number of reflections | 88292 | |
<I/σ(I)> | 24.3 | 3.9 |
Completeness [%] | 99.7 | 99.4 |
Redundancy | 5.9 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |