4B1J
Structure of human PARG catalytic domain in complex with ADP-HPD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-17 |
Detector | RIGAKU SATURN 944 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.848, 90.971, 94.763 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.630 - 2.080 |
R-factor | 0.17018 |
Rwork | 0.168 |
R-free | 0.21475 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4a0d |
RMSD bond length | 0.014 |
RMSD bond angle | 1.416 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 94.760 | 2.190 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.110 | 0.490 |
Number of reflections | 32176 | |
<I/σ(I)> | 12.4 | 2.8 |
Completeness [%] | 90.2 | 52.7 |
Redundancy | 6.3 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 25% PEG 3350, 36.4 MM PCTP-4, 63.6 MM PCTP-10, 0.2 M AMMONIUM SULPHATE |